Figure 6
Tas2r and canonical taste transduction pathway expression patterns in mouse gallbladder. In silico analysis of single-cell RNA sequencing data of murine gallbladder cells (data set GSM588894933). Unbiased clustering revealed 14 distinct cell clusters (0–13), among which cluster #9 represented tuft cells. Violin plots show expression of Tas2r in distinct cell clusters (A-F), Gnat3 (G), Plcb2 (H), Trpm5 (I) and Pou2f3 (J). Expression of Tas2r108 was restricted to cluster #0 (epithelium; 1 out of 9695 cells). Tas2r126 to clusters #1 (fibroblast; 1/7771) and 2 (mesenchyme; 1/3263). Tas2r135 to cluster #1 (7/7771), #2 (2/3263), #3 (SMC; 1/2457), #6 (endothelium; 4/1266), #7 (undefined; 1/1179), and #8 (mesenchyme; 1/1065). Tas2r137 to cluster #0 (1/9695). Tas2r138 to #0 (2/9695) and #9 (tuft cell; 1/852). Tas2r143 to #0 (12/9695), #1 (17/7771), #5 (SMC; 1/1359), #6 (4/1266), #7 (3/1179), #8 (1/1065), and #10 (lymphatic endothelium; 1/275).
