Figure 7
Tas2r expression patterns in mouse taste buds and gallbladder. In the vallate papilla from Pou2f3+/+ mice, expression of Pou2f3 (A), Tas2r108 (B), Tas2r126 (C), Tas2r135 (D), Tas2r137 (E), and Tas2r143 (F) was visualized by accumulation of black grains in radioactive in situ hybridization (ISH) experiments using anti-sense (as) riboprobes. ISH signals were absent from vallate papilla taste buds in mice lacking expression of Pou2f3 (Pou2f3–/–) (G–L). Tuft cells in the mucosal epithelium of the gallbladder from Pou2f3+/+ mice were detected by presence of Pou2f3 mRNA (M, arrows). No detectable expression was found for Tas2r108 (N), Tas2r126 (O), Tas2r135 (P), Tas2r137 (Q), and Tas2r143 (R). Likewise, no ISH signals were present in tissue samples from the gallbladder taken from Pou2f3–/– mice (S–X). Sections were counter-stained with methyl green. The luminal side (l) is always displayed up. e, epithelial layer; m, muscle layer. The scale bar in (A) equals 100 µm and applies to images A-K. The scale bar in (M) equals 50 µm and applies to images (M–X).
