Figure 8 | Scientific Reports

Figure 8

From: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors

Figure 8

Expression pattern of a TAS2R143-mCherry reporter mouse line. (A) Scheme depicting the generation strategy of the TAS2R143-mCherry transgenic mouse line. Native mCherry fluorescence is detectable in sections from vallate papilla taste buds (B), in epithelial cells at the squamo-columnar junction in the stomach (C), and in solitary cells in the gallbladder epithelium (E), but is absent from the epithelium of the duodenum (D) (representative images from n = 4 mice). Note some unspecific labeling of cell nuclei in lamina propria. Immunofluorescence for mCherry [arrows in (F)] overlaps with FLAP signals (G) in the gallbladder (F–H). The scale bar in (B) equals 100 µm, the scale bar in (C) equals 50 µm and also accounts for (D,E). The scale bar in (F) equals 25 µm and also accounts for (G,H).

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