Figure 6

Cfd KO in adipocytes reduced MMP9 expression in tumor cells. (A) Mmp2 and Mmp9 mRNA expression levels in EO771 cells or those cocultured with WT or Cfd KO mature adipocytes. Data are presented as mean ± SD. n = 3, *p < 0.05, **p < 0.01. (B) Activity of MMPs derived from EO771 cells cocultured with WT or Cfd KO mature adipocytes. Left, gelatin zymography; Right, densities of the bands for MMP2 and MMP9 were measured. Data are presented as mean ± SD. n = 3, *p < 0.05. (C) Visual abstract of the core findings of the study: mature adipocyte-cancer cell interaction promotes tumor invasion and metastasis at least partly by upregulating Lgals7 and MMP expression in the cancer cells.