Figure 6 | Scientific Reports

Figure 6

From: Renogrit selectively protects against cisplatin-induced injury in human renal tubular cells and in Caenorhabditis elegans by harmonizing apoptosis and mitophagy

Figure 6

Renogrit decreased cisplatin-induced cytosolic calcium accumulation and RIPK1-RIPK3-MLKL overexpression in HK-2 cells. (a) Image of Hoechst 33342 (nuclei, blue) and Fluo-4 AM (calcium, green) stained HK-2 cells induced with cisplatin at human Cmax (15 µg/ml) and treated with Renogrit (100 µg/ml) or NAC (2 mM). Magnification = 100 × . Scale bar (red) = 40 µm. Relative mRNA expression of genes involved in necroptosis namely (b) RIPK1, (c) RIPK2, and (d) MLKL of treated and untreated HK-2 cells. ### and ***, p < 0.001; ## and **p < 0.01; #p < 0.05.

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