Table 2 Deriving best fitted model of ‘Severity Score’ using the combinations of Ct values of different T cell markers in oro-nasopharyngeal swab RNA.

From: A novel metric-based approach of scoring early host immune response from oro-nasopharyngeal swabs predicts COVID-19 outcome

Principle of Ct value combinations

Non-severe Group (n = 10) Mean ∆Ct/∆Ctcombinations

Severe Group (n = 9)

Mean

∆Ct/∆Ctcombinations

Significance of difference: Severe vs non-severe COVID-19 cases by T-TEST

Pvalue

A. ΔCt values normalized with ACTB

T1- ACTB

12.61

13

0.7

T2- ACTB

3.67

3.07

0.20

T3- ACTB

13.87

13.2

0.56

T4- ACTB

6.47

9.49

0.01*

T5- ACTB

12.38

12.64

0.82

T6- ACTB

6.83

6.34

0.51

T7- ACTB

9.44

9.95

0.67

T8- ACTB

7.89

9.86

0.18

B. Composite ΔCt values by Combination of ACTB normalized ΔCt values

T4 + T2-2ACTB

10.14

12.56

0.07

T4 + T8-2ACTB

10.70

19.35

0.0027

T2 + T8-2ACTB

11.56

12.93

0.38

C. Composite ΔCt values by combinations of T cell phase specific markers

T4-T2

2.81

6.42

0.0022

T8-T2

4.22

6.79

0.093

T4-T8

− 1.42

− 0.37

0.60

T4 + T8-2.T2

7.03

13.21

0.0006

  1. ACTB: β-actin, Ct: cycle threshold of the individual target genes in RT-PCR, ΔCt: Difference of two Ct values as indicated, n: Number of samples, * P statistically significance at P < 0.05.
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