Fig. 6 | Scientific Reports

Fig. 6

From: Metformin enhances the therapeutic effects of extracellular vesicles derived from human periodontal ligament stem cells on periodontitis

Fig. 6

Met enhances the effects of EVs to ameliorate alveolar bone loss in SD rats with periodontitis. (A) Micro-CT images of alveolar bone loss (Scale bar: 1 mm). (B) Bone resorption was calculated as the distance from the buccal cementoenamel junction (CEJ) to the alveolar bone crest (ABC) (n = 6). (C) HE staining (Scale bar: 200 μm and 100 μm). (D) TRAP staining: Osteoclasts formation in different groups (Scale bar: 20 μm). (E) Quantitative analysis of TRAP-positive cells (n = 4). (F) Immunofluorescence staining for OCN (Scale bar: 20 μm). (G) Quantitative analysis of immunofluorescence staining for OCN (n = 6).). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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