Fig. 2
Mitochondrial dynamics in liver and muscle from nMig. to Mig. transition. Redheaded buntings were held under SD (nMig. condition) and transferred to LD on day 0. Liver and pectoralis muscles were isolated from nMig., and Mig. buntings held on day 16. Single cell suspension was prepared, followed by MitoTracker Red staining, followed by flow cytometry analysis. The flow cytometry histogram represents mitochondrial membrane potential (MMP+) during nMig. and Mig. day in liver (a,b) and muscle (g,h), respectively. Relative mRNA expression levels of various genes in the liver and muscle were quantified by SYBR Green method. Bar graphs represent relative mRNA expression levels of ACADM, PEPCK, and GOT2 genes in liver (c–e) and muscle (i–k) during nMig. and Mig. day and night. The expression levels of GLUT1 in liver and CS in muscles have been shown in graphs (f) and (l). Bar with cyan colour is for nMig. and red for Mig.; solid and pattern bar represent day and night sampling. Horizontal bar with asterisk represents groups with significant difference (p < 0.05, Tukey’s post hoc test). Data is presented as mean ± S.E.M. n = 6 in each group.
