Fig. 4
Apoptosis increased in liver nMig. to Mig., but decreased in muscles. Redheaded buntings were held under SD (nMig. condition) and transferred to LD on day 0. Liver and muscles were isolated from nMig., and Mig. buntings held on day 16. Single-cell suspensions of liver and muscles of nMig. and Mig. buntings were stained with Annexin V antibody and analysed by flow cytometry. The flow cytometry histogram represents apoptotic cells% in nMig. and Mig. day and night in liver (a–d) and muscle (i–l), respectively. Bar diagram represents relative mRNA expression levels for NF-κβ, CASP7, GPX1, and GPX4 in liver (e–h) and muscle (m-p) during nMig. and Mig. day and night. Bar with cyan colour is for nMig. and red for Mig.; solid and pattern bars represent day and night sampling. Horizontal bar with asterisk represents groups with significant difference (p < 0.05, Tukey’s post hoc test). Data is presented as mean ± S.E.M. n = 6 in each group.
