Fig. 4
Ex vivo histological analyses of inflammatory markers. (A, B) Mice treated two times with 01BSUR showed a significant lower expression of CD68 + macrophages (p < 0.05) and interleukin-1β (p < 0.05) in atherosclerotic plaque area after 8 weeks of high-fat diet compared to mice of the control group. (C) Immunofluorescence analyses: Macrophages (CD68 +) are shown in green, interleukin-1β in red and cell nuclei (DAPI) in blue. The stacked image shows a co-localization of interleukin-1β with macrophages. (D) The amount of CD68 + macrophages and IL-1β-expression within the atherosclerotic plaque area were in good correlation (y = 1.22x + 0.35; R2 = 0.87). *indicates the arterial lumen; #indicates the plaque area; Scale bars represent 100 µm.
