Fig. 1

CSE is highly expressed in ER+ breast cancer cells and negatively correlates with TAM sensitivity. A Expression levels of CSE proteins in two types of ER+ breast cancer cells and normal breast cells. MCF7 and T47D are ER+ breast cancer cell lines. Hsbst578 is a normal breast cell line. All data are expressed as mean ± standard deviation (n = 3). ^**P < 0.01 versus Hsbst578 cells; ^##P < 0.01 versus T47D cells. (B and C) MTS method was used to detect the sensitivity and IC₅₀ value of MCF7 and T47D cells to 4-OH TAM. All data are expressed as mean ± standard deviation (n = 3). ^*P < 0.05 versus MCF7 cells. (D–G) The Western blot assay was used to detect the expression levels of CSE proteins in MCF7 and T47D cells as the duration and concentration of 4-OH TAM action increased. All data are expressed as mean ± standard deviation (n = 3). ^*P < 0.05, ^**P < 0.01 versus 0 μM 4-OH TAM group; ^#P < 0.05, ^##P < 0.01 versus 0 h group. (H–K) The methylene blue method was used to detect the level of H2S in MCF7 and T47D cells as the duration and concentration of 4-OH TAM action increased. All data are expressed as mean ± standard deviation (n = 3). ^*P < 0.05 versus 0 μM 4-OH TAM group; ^#P < 0.05 versus 0 h group.