Fig. 3 | Scientific Reports

Fig. 3

From: Cystathionine-γ-lyase contributes to tamoxifen resistance, and the compound I194496 alleviates this effect by inhibiting the PPARγ/ACSL1/STAT3 signalling pathway in oestrogen receptor-positive breast cancer

Fig. 3

Overexpression of CSE protein in T47D cells can increase the inhibitory effect of TAM on the growth, proliferation, migration and invasion of breast cancer cells. (A and B) Analysis of the efficiency of overexpression of CSE proteins in T47D cells. (C–E) MTS and EdU assays were used to detect the effect of 4-OH TAM on the growth and proliferation of T47D cells after upregulation of CSE, respectively. Representative images were taken. Scale bars, 100 µm. (F and G) Wound healing assay was used to detect the effect of 4-OH TAM on the migration of T47D cells after upregulation of CSE. Representative images were taken. Scale bars, 500 µm. (H–J) Transwell assay was used to determine the effect of 4-OH TAM on the migration and invasion of T47D cells after upregulation of CSE. Representative images were taken. Scale bars, 200 µm. All data are expressed as mean ± standard deviation (n = 3). *P < 0.05, ***P < 0.001 versus the Vector group.

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