Fig. 4

Knocking down the expression of CSE in MCF7/TAM cells can increase the inhibitory effect of TAM on the growth, proliferation, migration and invasion and the apoptosis-promoting effect of breast cancer cells. (A and B) The MTS method was performed to detect the sensitivity and IC₅₀ of MCF7 and MCF7/TAM cells to 4-OH TAM. All data are expressed as mean ± standard deviation (n = 3). ^*P < 0.05 versus MCF7 cells. (C and D) The expression levels of CSE proteins in MCF7 and MCF7/TAM cells. All data are expressed as mean ± standard deviation (n = 3). ^***P < 0.001 versus MCF7 cells. (E and F) Analysis of the efficiency of knocking down CSE proteins in MCF7/TAM cells. All data are expressed as mean ± standard deviation (n = 3). ^*P < 0.05 versus the Sc siRNA. (G–I) MTS and EdU assays were used to detect the effect of 4-OH TAM on the growth and proliferation of MCF7/TAM cells after CSE knockdown, respectively. Representative images were taken. Scale bars, 100 µm. (J) Wound healing assay was used to detect the effect of 4-OH TAM on the migration of MCF7/TAM cells after CSE knockdown. Representative images were taken. Scale bars, 500 µm. (K) Transwell assay was used to determine the effect of 4-OH TAM on the migration and invasion of MCF7/TAM cells after knockdown CSE expression. Representative images were taken. Scale bars, 200 µm. (L) Apoptosis assay was used to detect the effect of 4-OH TAM on apoptosis of MCF7/TAM cells after knockdown of CSE expression. Representative images were taken. All data are expressed as mean ± standard deviation (n = 3). ^*P < 0.05, ^**P < 0.01 versus 0 μM 4-OH TAM group.