Fig. 5 | Scientific Reports

Fig. 5

From: Cystathionine-γ-lyase contributes to tamoxifen resistance, and the compound I194496 alleviates this effect by inhibiting the PPARγ/ACSL1/STAT3 signalling pathway in oestrogen receptor-positive breast cancer

Fig. 5

CSE regulates the transcription of ACSL1 through PPARγ to affect the expression and activity of STAT3 protein, thereby participating in TAM resistance in ER+ breast cancer. (A and B) Expression levels of ACSL1 protein in MCF7/TAM resistant cells of ER+ breast cancer. All data are expressed as mean ± standard deviation (n = 3). *P < 0.05 versus MCF7 cells group.**P < 0.01 versus MCF7 cells group. (C and D) The effect of down-regulation of CSE on the expression levels of ACSL1, STAT3 and p-STAT3 in MCF7/TAM cells. All data are expressed as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01 versus Sc siRNA group. (E and F) The effect of downregulation of CSE protein on the palmitoylation level of STAT3 in MCF7/TAM cells. The acyl-biotin substitution method was applied to detect STAT3 palmitoylation. All data are expressed as mean ± standard deviation (n = 3). **P < 0.01 versus Sc siRNA group. (G and H) Effect of down-regulation of ACSL1 protein on STAT3, p-STAT3 expression in MCF7/TAM. All data are expressed as mean ± standard deviation (n = 3). *P < 0.05 versus Sc siRNA group. (I and J) Effect of down-regulation of ACSL1 protein on STAT3 palmitoylation in MCF7/TAM. All data are expressed as mean ± standard deviation (n = 3). *P < 0.05 versus Sc siRNA group.

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