Fig. 9

I194496 can reverse CSE-regulated PPARγ/ACSL1/STAT3 pathway signaling in T47D/CSE and MCF7/TAM of breast cancer cells. (A–D) Effect of I194496 in combination with TAM on PPARγ, ACSL1, STAT3, p-STAT3, and CSE protein expression in T47D/CSE and MCF7/TAM cells. (E and F) Effect of I194496 in combination with TAM on PPARγ binding activity to the ACSL1 promoter in T47D/CSE and MCF7/TAM cells. The overexpression plasmids of ACSL1 promoter and PPARγ were cotransfected into T47D/CSE and MCF7/TAM cells, and then assayed by applying a dual luciferase reporter gene assay kit. (G–J) Effect of I194496 in combination with TAM on STAT3 palmitoylation in T47D/CSE and MCF7/TAM cells. The acyl-biotin substitution method was applied to detect STAT3 palmitoylation. All data are expressed as mean ± standard deviation (n = 3). *P < 0.05 versus Control group. #P < 0.05 versus 4-OH TAM (12 μM) group.