Fig. 7 | Scientific Reports

Fig. 7

From: Prognostic significance and response to immune checkpoint inhibitors of RIPK3, MLKL and necroptosis in non-small cell lung cancer

Fig. 7

The impact of RIPK3 expression in NSCLC cells on T-cell responsiveness to immune checkpoint inhibitors: (A) Western blot analysis of RIPK3 and PD-L1 expression in NSCLC cells. (B) Western blot analysis demonstrating RIPK3 expression in H1975 cell lines following stable lentiviral transduction. (C) Bar graph illustrating the percentage of cell death in H1975-expressing RIPK3 cells induced by necroptosis stimuli. (D) Illustration displaying the experimental workflow for co-culture between NSCLC cells and PBMC-derived T cells. (E) Bar graph representing the percentage of T cell-mediated cell death in H1975-expressing RIPK3 cells in the presence or absence of pembrolizumab. Data represent three independent experiments ± SD. (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001); ns non-significant.

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