Fig. 1 | Scientific Reports

Fig. 1

From: Heterogeneous expression of the atypical chemokine receptor ACKR3 in glioblastoma patient-derived tissue samples and cell cultures

Fig. 1

ACKR3 tool validation using flow cytometry. (A) Cell surface ACKR3 expression in U87, U87 ACKR3 and U87 CXCR4 cell lines by using 8F11-M16 and 11G8 antibodies. (B) Immunofluorescent staining of ACKR3 (green) in U87 and U87 ACKR3 cells using 11G8 MAB42273 antibody against ACKR3. DAPI (blue) was used to counterstain nuclei. Scale bar = 50 μm. (C) Western Blot (WB) analysis of ACKR3 in protein extracts from U87, U87 ACKR3, T033-mRFP, T033-hACKR3 and MCF-7 cells, using different commercially available antibodies (11G8/MAB42273, ab72100, ACR-037 and 7TM0080N). Original blots/gels are presented in Supplementary Figure S3.

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