Fig. 4 | Scientific Reports

Fig. 4

From: Heterogeneous expression of the atypical chemokine receptor ACKR3 in glioblastoma patient-derived tissue samples and cell cultures

Fig. 4

ACKR3 surface expression in patient-derived GSCs is low and remains unchanged upon CXCL11 or CXCL12 stimulation. (A) Phase-contrast images of patient-derived GSCs (T08, T013, T018 and T033) cultured as 3D tumorospheres (scale bar = 100 μm). (B) ACKR3 mRNA expression relative to GAPDH mRNA expression in patient-derived GSCs and U87 cells (U87 overexpressing ACKR3 were used as positive control) (n = 4). (C) Fluorescence intensity of ACKR3 membrane staining in patient-derived GSCs and U87 cells using flow cytometry (U87 overexpressing ACKR3 were used as positive control) (n = 4). (D) Percentage of ACKR3 positive cells in patient-derived GSCs using flow cytometry. (E–F) ACKR3 and CXCR4 fluorescence intensity (and % of positive T018 GSCs) after CXCL11 or CXCL12 stimulation for 24–48 h (n = 4). Statistical significance was determined by one-way ANOVA (∗ p < 0.05).

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