Fig. 6 | Scientific Reports

Fig. 6

From: Heterogeneous expression of the atypical chemokine receptor ACKR3 in glioblastoma patient-derived tissue samples and cell cultures

Fig. 6

(A) T033-RFP-LUC cells were engrafted in the right striatum of nude mice, sacrificed 42 days post-engraftment. Two different brain regions were isolated (“SVZ in” and “SVZ out”) and cells were cultured for 7 days before RT-qPCR analyses were performed. (B) Quantitative RT-qPCR analysis allowed to detect ACKR3 mRNA expression normalized to HKG (GAPDH, EF1a, HPRT) in T033 (in vitro), T033 “SVZ in” (in vivo) and T033 “SVZ out” (in vivo). Statistical significance was determined by one-way ANOVA (*** p < 0.001, **** p < 0.0001). (C) ACKR3 mRNA expression relative to Ezrin used as HKG in T033 GSCs vs. T033 GSCs stimulated with CXCL12 (10nM).

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