Fig. 4
LMK235 attenuated interstitial fibrosis in the border zone 21 days after MI, and LMK235 inhibited upregulation of LSD1 in the border zone 21 days after MI. A-D & F, Representative images of Masson’s trichrome staining in each group at magnifications of 40× and 100× and statistical analysis of the fibrotic area (n = 3 for each group). E & G-K, Western blot results and statistical analysis of collagen I, collagen III, LAP-TGF-β1 and LSD1 expression (n = 5 for sham group and sham + LMK235 group, while n = 7 for MI group and MI + LMK235 group). The band of collagen I, LAP-TGF-β1 and GAPDH (Fig. 4E) were from the same gel, the band of LSD1 and GAPDH (Fig. 4J) were from the same gel, and the band of collagen III were from the other gel. The data are presented as the mean ± SD, *P < 0.001 vs. the sham group, #P < 0.001 vs. the MI group. The samples derive from the same experiment and that blots were processed in parallel. The original blots are presented in Supplementary Figs. 17–23.
