Fig. 5
LMK235 suppressed LPS-induced inflammatory macrophage polarization and inflammatory cytokine expression by inhibiting NF-κB pathway in RAW264.7 cells, while overexpression of LSD1 abrogated the anti-inflammatory effect of LMK235. A-B, Flow cytometry results and statistical analysis of the percentage of CD86+ macrophages (n = 4 for each group). C-J, Western blot results and statistical analysis of IL-6, TNF-α, LSD1, p-p65 and p65 expression in RAW264.7 cells in each group. n = 4 for each group. The band of IL-6 and GAPDH (Fig. 5C) were from the same gel, the band of TNF-α were from the other gel. The band of LSD1, t-p65 and GAPDH (Fig. 5F) were from the same gel, and the band of p-p65 were from the other gel. The data are presented as the mean ± SD, *P < 0.001 vs. the NC group, #P < 0.01 vs. the LPS group, ☨P < 0.01 vs. the LPS + LMK235 group. The samples derive from the same experiment and that blots were processed in parallel. The original blots are presented in Supplementary Figs. 24–32.
