Fig. 6
LMK235 suppressed TGF-β1-induced fibroblast-myofibroblast transformation by inhibiting Smad2/3 pathway in MCFs, while overexpression of LSD1 abrogated the antifibrotic effect of LMK235. A‒H, Western blot results and statistical analysis of collagen I, collagen III, α-SMA, LSD1, p-Smad2, Smad2, p-Smad3 and Smad3 expression in MCFs in each group. n = 4 for each group. The band of collagen I, α-SMA and α-tublin (Fig. 6A) were from the same gel, the band of collagen III were from the other gel. The band of LSD1, t-Smad2 and α-tublin (Fig. 4J) were from the same gel, and the band of p-Smad2, p-Smad3 and t-Smad3 were from the other three gels. The data are presented as the mean ± SD, *P < 0.001 vs. the NC group, #P < 0.01 vs. the TGF-β1 group, ☨P < 0.01 vs. the TGF-β1 + LMK235 group. The samples derive from the same experiment and that blots were processed in parallel. The original blots are presented in Supplementary Figs. 33–46.
