Fig. 5 | Scientific Reports

Fig. 5

From: Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2

Fig. 5

Virus titration and serum neutralization assays using Vero E6-TMPRSS2-T2A-ACE2 cells. (A) Titration of the indicated SARS-CoV-2 strains on VeroE6/TMPRSS2 or Vero E6-TMPRSS2-T2A-ACE2 cells. The viral titer was determined in a TCID50 assay. (B) Neutralization assay using anti-RBD serum specific for the SARS-CoV-2 ancestral and BA.5 was performed on VeroE6, VeroE6/TMPRSS2, and Vero E6-TMPRSS2-T2A-ACE2 cells. The neutralization (NT) titers, defined as the geometric mean of the reciprocal of the highest sample dilution that protected from CPE (using duplicate wells for each measurement) are shown.

Back to article page