Fig. 3 | Scientific Reports

Fig. 3

From: Assessment of NK cytotoxicity and interactions with porcine endothelial cells by live-cell imaging in 2D static and 3D microfluidic systems

Fig. 3

2D live-cell tracking allows analysis at a single-cell level and reveals cell death pathways. Two conditions were tested; PAECs overnight activated with porcine tumor necrosis factor (pTNF) or not (Φ). Colors represent PAECs’ nuclei (blue), cells with disrupted membranes (pink), NK cells (yellow). (A) Pictures showing apoptotic and necrotic cell death of pTNF-activated PAECs lysed by NK cells were captured during a 20 min time-lapse video using an effector-to-target (E:T) ratio of 1:1. Scale bar = 50 μm. (B) Quantification of PAEC death occurring by apoptosis and necrosis after 120 min interaction with human NK cells at E:T ratio = 1:1. Nested-plot for the percentage of apoptotic and necrotic cells in total counted adherent PAECs, in control and pTNF-treated targets (left), and bar-plot comparing differences in apoptosis within the dead PAEC population between control and pTNF-treated PAECs (right). Data were from three different healthy donors, analyzed by paired t-test.

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