Fig. 1 | Scientific Reports

Fig. 1

From: GCC2 promotes non-small cell lung cancer progression by maintaining Golgi apparatus integrity and stimulating EGFR signaling pathways

Fig. 1Fig. 1

GCC2 knockdown inhibits NSCLC proliferation, migration, invasion, and survival. (A) RT-qPCR analysis and (B) western blot analysis of relative GCC2 expression levels in H460 cells following transfection with shGCC2. (C) Cell viabilities of GCC2 knockdown H460 cells were measured by MTS assay at 0, 4, 8, 12, and 24 h. (D) Detection of TUNEL-positive cells in H460 cells transfected with shGCC2 plasmids. Scale bar = 50 μm. RT-qPCR analysis of relative gene expression of (E) CSC-related biomarkers and (F) EMT-related genes. (G) Colony formation assays of control and GCC2 knockdown H460 cells with colony counts summarized on the right. (H) Wound-healing assay measured at 0 and 48 h demonstrating the migratory potential of control and GCC2 knockdown H460 cells. Scale bar = 100 μm. (I) Transwell migration and invasion assays in control and GCC2 knockdown H460 cells. Scale bar = 100 μm. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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