Fig. 3

GCC2 knockdown decreases various receptor tyrosine kinase expression and downstream MAPK signaling pathway. (A) RT-qPCR analysis of various receptor tyrosine kinases in control and GCC2 knockdown H460 cells. (B) Western blot analysis of MEK, pMEK, ERK, pERK of whole-cell lysates of control and GCC2 knockdown H460 cells. (C) RT-qPCR analysis of MAPK signaling pathway-related genes in control and GCC2 knockdown H460 cells. (D) Western blot analysis of cyclin D1 in whole-cell lysates of control and GCC2 knockdown H460 cells. Immunofluorescence analysis of (E) cyclin D1 (scale bar = 50 μm) and (F) Ki-67 (scale bar = 10 μm) in control and GCC2 knockdown H460 cells with quantification of CCDN1 + and Ki-67 + cells, respectively, relative to Hoechst stained cells. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.