Fig. 4
GCC2 knockdown inhibits H460 tumorigenesis in vivo. BALB/c nude mice were subcutaneously injected with 1 × 106 GCC2 knockdown or control H460 cells and were sacrificed 21 days after tumor inoculation (n = 5 for each group). (A) Photograph of dissected tumor xenografts from the indicated experimental groups. Tumor analysis for (B) tumor volumes, which were recorded 3 times a week, and (C) tumor weights, which were measured immediately after tumor extraction. (D) Bodyweights of mice were measured over the course of the experiment. (E) Representative images of hematoxylin–eosin stained tumor paraffin sections. Scale bar = 50 μm. (F) Immunohistochemistry analysis for EGFR and cyclin D1 in control and GCC2 knockdown H460 tumor xenografts. Scale bar = 10 μm. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
