Fig. 3
Comparative nanoparticle tracking analysis (NTA) of CSF-derived particles, extracellular vesicles (EVs). Panel (a) Results of NTA performed on raw-CSF (blue) and trapped (green)of a cognitively unimpaired (CU) patient. The CSF was subsequently diluted to a total volume of 1 ml in preparation for NTA. The mean particle size is 127 nm and the mode is 83 nm. Size distribution of trapped EVs from CSF. The results, aggregated from five separate captures, show that the trapped samples have a mean particle size of 145 nm mode 93 nm. The graphical profiles display an NTA analysis of EVs based on three 90-second videos capturing the trapped EVs. (b) Effects of different wash flow rates on EV yield using NTA. The figure shows the NTA of EVs isolated from the CSF of a healthy control subject. The EVs were trapped using two distinct washing flow rates, set to 200 µl/min or 30 µl/min, to evaluate the impact of flow rate on EV capture efficiency. In each condition, the trapping procedure was performed in triplicate, and the resultant EVs from these three runs were combined. The pooled samples were then diluted to a final volume of 1 ml to facilitate NTA measurement of the concentration of particles and different wash flow rates.
