Fig. 6
Evaluation of EVs Lysis Methods from Trapped CSF. This analysis was conducted on EVs isolated from the CSF of a healthy control subject, following washing at a flow rate of 200 µl/min. Illustrates particle distribution profiles derived from NTA after applying various EV lysis techniques. The methodologies tested included Triton X at 1% 25 min incubation (Blue), Tween 20X at 0.5% (Yellow), and a mechanical lysis method, Bioruptor, performed for 20 cycles (alternating 30 s off and on) (Green). Each lysis method was assessed for its impact on particle distribution, with results indicating distinctive effects from each treatment compared to the control sample (Red).
