Fig. 2 | Scientific Reports

Fig. 2

From: CIGB-300 internalizes and impairs viability of NSCLC cells lacking actionable targets by inhibiting casein kinase-2 signaling

Fig. 2

Short-term internalization of CIGB-300 peptide in live NSCLC cells as analyzed by flow cytometry. (a) Representative FSC vs. SSC dot-plots (upper panel) and FL1-Histograms (lower panel) obtained after incubation of A549 cells with 30 μm or an equipotent concentration (IC50) of a peptide-fluorescein conjugate during 3, 10, 30, 45 and 60 min. Live cells on FSC vs. SSC plots were selected to estimate the Geometric mean of the fluorescence on FL1 channel in arbitrary units (GmeanF, a.u.). Vehicle-treated cells were used to set an auto-florescence threshold to score per cent of cells displaying florescence (%CIGB-300-F+). (b) Estimation of %CIGB-300-F + cells (upper-panel) and magnitude of peptide internalization (GmeanF, a.u., lower-panel) for the four cell lines in study (n = 3). (c) The collected GmeanF values (a.u) were fitted to a first-order exponential association model to obtain the kinetic parameters and confidence intervals described in the Table; Ymax, maximum Fluorescence at the curve plateau; K, rate constant of internalization; Half-time = ln(2)/K; R square, Goodness of fit.

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