Fig. 6 | Scientific Reports

Fig. 6

From: CIGB-300 internalizes and impairs viability of NSCLC cells lacking actionable targets by inhibiting casein kinase-2 signaling

Fig. 6

ROS detection by flow cytometry after incubation of LUAD cells with CIGB-300 as revealed by DHE method. (a) Representative analysis with FSC vs. SSC and histograms plots (Overlays) corresponding to A549 cells incubated during 6 h with CIGB-300 at IC50 concentration value; In the overlay histograms plots a DHE high population emerged on CIGB-300 incubated cells, with mean Fluorescence values (GMeanF) in arbitrary units (a.u) showed as inserts. (b) Quantification of GmeanF on A549 cells treated as (a); (c) Quantification of GmeanF on NCI-H522 cells incubated during 6 h with IC50 concentration. Veh, vehicle; NAC, N-acetyl cysteine. Selected groups were compared against Veh using an Unpaired t-test (two-tailed) and statistical differences, when found, denoted as: *, p < 0.05; **,p < 0.01; ***, p < 0.001. Square ** refers to statistical analysis for ROS quenching effects from NAC, i.e. CIGB-300 vs. CIGB-300 + NAC groups; H2O2 vs. H2O2 + NAC.

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