Figure 4
Phenotype of dopaminergic neurons in necdin deficient mice. (a) Expression of necdin (green) and TH (red) in the VTA using immunofluorescence staining. Scale bar, 50 μm. (b) Representative images of midbrain TH immunofluorescence in 3-month-old WT and KO mice. Scale bar, 100 μm. (c) Quantification of DA cell number in VTA. (d) Representative images of midbrain TH immunofluorescence in 13-month-old WT and KO mice. Scale bar, 100 μm. (e) Quantification of DA cell number in VTA. Data were mean ± SEM, n = 5, *p < 0.05, **p < 0.01, unpaired t-test.
