Fig. 7

Polydeoxyribonucleotide (PDRN) downregulates the NF-κB signaling pathway under IL-1β-induced inflammation. (A) Expression of NF-κB signaling pathway mediators in human bone marrow-derived mesenchymal stem cells (hBMSCs) treated with IL-1β (10 ng/mL) in the presence or absence of PDRN (100 µg/mL) was evaluated using western blot analysis. (B) Densitometry analysis of protein bands shown in (A) using the ImageJ software. P-values were determined using a one-way analysis of variance with Bonferroni post hoc tests. (C) Immunofluorescence images showing nuclear translocation of p65 in hBMSCs treated with IL-1β (10 ng/mL) in the presence or absence of PDRN (100 µg/mL). Nuclei were counterstained with DAPI. Scale bar = 20 μm. Results are means ± SDs. n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001.