Fig. 2
From: Targeted imaging of pulmonary fibrosis by a cyclic peptide LyP-1
LyP-1 colocalizes with extracellular components such as collagen I. Mice were intravenously injected with FITC-labeled ARA or LyP-1 peptides at day 18 after BLM instillation. Peptides were allowed to circulate for 2 h, and lungs were harvested for frozen sections. The sections were stained for cell markers (shown in red) including F4/80 (a) and CD45 (b), anti-P32 (c), the cytoskeleton marker phalloidin (d), collagen I (e), and peptides using anti-fluorescein antibody (anti-FITC; shown in green) and were counterstained with 4,6-diamidino-2-phenylindole (DAPI; shown in blue). Immunofluorescence images showed the localization of FITC-LyP-1 in the fibrotic region of lung sections from BLM mice. The threshold was equally enhanced for all samples using ImageJ. Representative sections of ARA or LyP-1 peptide binding to lung tissue of BLM mice are shown (n = 4 ~ 6 mice per group), with scale bars of 25 µm for the full field view. For magnified (zoom-in) areas, scale bars represent 10 µm.
