Fig. 3

MitoQ Enhances ACSL1 and PINK1/Parkin-Mediated Mitophagy in Rat Lungs Following BLM Exposure. (A) TEM images illustrating mitochondrial morphology. (B) The ratio of the long diameter to the short diameter of mitochondria. (C) Western blot (WB) analysis of Drp1, Mfn2, BECN1, and p62 expression, with β-actin used as a loading control. (D) Quantification of WB analysis. (E) Immunohistochemistry (IHC) staining for ACSL1. (F) Comparison of ACSL1 levels across different groups. (G) IHC staining for PINK1. (H) Comparison of PINK1 levels across different groups. (I) IHC staining for Parkin. (J) Comparison of Parkin levels across different groups. (K) WB analysis of ACSL1, PINK1, and Parkin expression, with β-actin used as a loading control. (L) Quantification of WB analysis. Abbreviations: ACSL1, long-chain fatty acyl-CoA synthetase 1; BECN1, Beclin-1; BLM, bleomycin; Drp1, dynamin-related protein 1; IHC, immunohistochemistry; Mfn2, mitofusin 2; PINK1, PTEN-induced putative kinase 1; Parkin, E3 ubiquitin ligase Parkin; TEM, transmission electron microscopy; WB, western blotting. Data are expressed as mean ± SEM, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001.