Fig. 5
MitoQ improved redox damage, mitochondrial dysfunction, and PINK1/Parkin-mediated mitophagy in A549 cell lines exposed to BLM. (A) Comparison of cell viability among the different groups. (B) Comparison of GSH concentrations among the different groups. (C) Comparison of SOD concentrations among the different groups. (D) Comparison of MDA concentrations among the different groups. (E) Comparison of fluorescence intensity of ROS among the different groups. (F) Comparison of fluorescence intensity ratio of JC-1 monomers to JC-1 aggregates among the different groups. (G) Observation of DCFH-DA staining, scale bar: 50 μm, magnification: 20x. (H) Observation of JC-1 staining, scale bar: 20 μm, magnification: 40x. (I) Colocalization analysis of LysoTracker (Green) and MitoTracker (Red) staining. (J) Expression levels of α-SMA, FN1, Drp1, Mfn2, BECN1, p62, ACSL1, PINK1, and Parkin measured by WB assays, with β-actin used as a loading control. (K) Quantification of WB analysis. Abbreviations: BLM, bleomycin; Drp1, dynamin-related protein 1; GSH, glutathione; IHC, immunohistochemistry; MDA, malondialdehyde; Mfn2, mitofusin 2; PINK1, PTEN-induced putative kinase 1; Parkin, E3 ubiquitin ligase Parkin; SOD, superoxide dismutase; ROS, reactive oxygen species; WB, western blotting. Data are expressed as mean ± SEM, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001.
