Fig. 2
From: Rac1 inhibition regenerates wounds in mouse fetuses via altered actin dynamics
Effect of NSC23766 treatment on mouse epidermal keratinocyte PAM212. (a) Actin dynamics at the PAM212 cell margin in a scratch assay (immunocytochemistry). Scale bar = 10 μm. Red: E-cadherin; green: actin; blue: DAPI (nuclei). Yellow arrowheads: filopodia. (b) Expression of Rac1 signaling-related molecules in response to NSC23766 treatment. *P < 0.05. (c) Changes in Rac1 activity in response to NSC23766 treatment (Rac1 activation assay). *P < 0.05. (d) Quantitative comparison of Rac1 signaling gene expression after NSC23766 treatment. *P < 0.05. (e) Effect of NSC23766 treatment on cell migration capacity in the scratch assay. The area covered by migrating cells was measured as the area of migration relative to the area of the gap due to scratching. *P < 0.05.
