Fig. 5
From: Rac1 inhibition regenerates wounds in mouse fetuses via altered actin dynamics
Wound healing in fetuses of epidermis-specific Rac1-knockout mice (K14-CreERT2;Rac1flox/flox). (a) Macroscopic analysis of scars in embryonic day (E)14 fetuses 72 h after wounding. The wound was completely regenerated in the tamoxifen group. Yellow dotted line: extent of visible mark. Scale bar = 1 mm. (b) Quantitative comparison of wound depth and area 72 h after wounding at E14. *P < 0.05. (c) Immunostaining for actin in the wound epidermis at E14 and 36 h after wounding. Green: actin; blue: DAPI (nuclei); arrows: areas where actin is expressed in a cable-like structure. Scale bar = 20 μm. (d) Protein expression of Rac1-signaling-related molecules in E14 embryos from epidermis-specific Rac1-knockout mice. *P < 0.05. (e) Changes in Rac1 activity in fetal E14 epidermis-specific Rac1-knockout mice (Rac1 activation assay). *P < 0.05.
