Fig. 6

Unsupervised hierarchical clustering based on genes related to PCD. (A-B) HCC samples were clustered in three robust subtypes by NMF algorithm in the TCGA cohort using1000 iterations when the optimal cluster number k = 3. The homogeneity of red-coloring and average silhouette seen in the graphs indicate the presence of 3 clusters of HCC patients. (C) Visualization of cluster results using t-SNE analysis. The three clusters were separated from each other (D) Heatmaps of differential DEGs amomg three subtypes. (E) Survival analysis revealed prognostic differences (right, OS; left, RFS) between three HCC subtypes.