Table 1 Primers used in the study.

Gene	Primer Sequence 5’ to 3’	Annealing temperature	References
TiPV-F	GAGATGGTGTGAAAATGAACGGG	60 °C	⁸
TiPV-R	CTATCTCCTCGTTGCTCGGTGTATC	60 °C
TiPV-Fq	GCACCACAGCTGAGTACAAC	56 °C
TiPV-Rq	AACTGCTCGGCTATCTCCTC	56 °C
β-Actin-F	CAGCAAGCAGGAGTACGATGAG	60 °C	⁶¹
β-Actin-R	TGTGTGGTGTGTGGTTGTTTTG	60 °C
IL8-F	GCACTGCCGCTGCATTAAG	60 °C
IL8-R	GCAGTGGGAGTTGGGAAGAA	60 °C
TLR7-F	TCAGCAGGGTGAGAGCATAC	60 °C
TLR7-R	ACATATCCCAGCCGTAGAGG	60 °C
MHC-II-F	TGGCCCTGACTGAACCACTG	60 °C
MHC-II-R	TCAGACCCACGCCACAGAAC	60 °C
NFκB-F	AACGACGGTGATGACAACGAC	60 °C
NFκB-R	AAATTCAGGCTCCACACTGACC	60 °C
CRs- F	ACAGAGCCGATCTTGGGTTACTTG	60 °C
CRs- R	TGAAGGAGAGGCGGTGGATGTTAT	60 °C
IL1β -F	TGCACTGTCACTGACAGCCAA	60 °C
IL1β -R	ATGTTCAGGTGCACTATGCGG	60 °C
TNF-α -F	CCAGAAGCACTAAAGGCGAAGA	60 °C
TNF-α -R	CCTTGGCTTTGCTGCTGATC	60 °C

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