Fig. 3
From: The deleterious effects of CDK4/6 inhibition on renal recovery post-acute kidney injury
Palbociclib administration post-acute stage of AKI promotes renal senescence and fibrosis. Palbociclib was administered to C57BL/6 mice via gavage from day 8 to day 14, and the mice were euthanized on day 21 post-renal ischemia (A). Relative mRNA expression levels of P16 and P21 are shown (B, C). Relative mRNA expression levels of Collagen I and Fibronectin are depicted (D, E). Representative immunohistochemical staining of α-SMA, Collagen I, and Fibronectin in renal tissue (scale bar, 50 μm) is presented (F), along with semi-quantitative analysis of α-SMA, Collagen I, and Fibronectin positive areas in renal tissue (H, I, J). Representative immunohistochemical staining of F4/80 (scale bar, 50 μm) is shown (G), and semi-quantitative analysis of macrophage infiltration in renal tissue is provided (K). The qPCR results are shown as the fold change compared with the Veh group. Data are expressed as mean ± SD. n = 4 mice. *p < 0.05, **p < 0.01, ***p < 0.001.
