Fig. 7

Optimization of plasmid PET22b-E. coli in conditions (Calculated with a conversion rate of 100% under optimal single-factor conditions). (a) Effect of penetrant on enzyme activity. (b) Effect of Incubation temperature on enzyme activity. (c) Effect of Incubation pH on enzyme activity. (d) Effect of substrate concentration on enzyme activity. (e) Effect of bacterial concentration on enzyme activity. (f) Effect of incubation time on enzyme activity. ns, no significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are expressed as the mean ± standard deviation (SD) of 3 independent experiments.