Table 1 Strains and plasmids used in this study.
Strains or plasmids | Relevant characteristics | Sources |
|---|---|---|
Strains | ||
B. subtilis 168(168) | Stored in our lab | |
WB600 | (B. subtilis 168) ΔnprE, ΔaprA, Δepr, Δbpr, Δmpr, ΔnprB, ΔamyE Resistance to Erythromycin | Stored in our lab |
+bslA | WB600 with the overexpression of bslA | This study |
ΔbslA | WB600 with the deletion of bslA | This study |
WB600-bslA-cgt | cgt comes from plasmid pUC57 | This study |
WB600-cgt | Free expression of β-CGTase in strain WB600 | This study |
Plasmids | ||
pBE2R | Resistance to Ampicillin | Stored in our lab |
pJOE8999 | Resistance to Ampicillin | Stored in our lab |
pUC57 | Resistance to Kanamycin | Stored in our lab |
