Fig. 2
From: WWP1 targeting PTEN for polyubiquitination to promote bone metastasis of luminal breast cancer
WWP1 interacts with PTEN in luminal breast cancer cell lines. (A) Western blot analysis of WWP1 expression levels in different breast cancer cell lines. (B,C) Quantifications of WWP1 levels relative to GAPDH among different breast cancer cell lines are shown. (D) BT474, MCF7, and T47D cells transfected with Flag-WWP1 were subjected to immunoprecipitation using anti-Flag antibodies. Both cell lysates and immunoprecipitates were then blotted. (E–G) Interaction between WWP1 and PTEN in MCF7 cells, T47D cells, and BT474 cells was confirmed through Co-IP assays. Cell lysates were immunoprecipitated using control IgG, anti-WWP1, and anti-PTEN antibodies. The immunoprecipitates were then analyzed.
