Fig. 6
From: Jararaca GPIb-binding protein causes thrombocytopenia during Bothrops jararaca envenomation
Platelet aggregation tracings induced by fractions D or E. (a) Mouse whole blood: 400 µL of citrated BALB/c mouse blood was diluted in 400 µL of saline. (b) Human whole blood: 400 µL of human citrated blood was diluted in 400 µL of saline. At the time indicated by the asterisk, 20 µL of PBS (control), protein fraction E (61 µg/mL) or fraction D (35 µg/mL) were added. Collagen (Col, final concentration 4.9 µg/mL for human platelets, or 12 µg/mL for mouse platelets) was used as a positive control. (c–e) Platelet aggregation in human PRP induced by the platelet agonists ristocetin ((c) 1.5 mg/mL, final concentration), collagen ((d) 4.5 µg/mL, final concentration), or PAR-1 agonist ((e) SFFLRN, 30 µM, final concentration). Aliquots of 40 µL of fraction E (61 µg/mL), fraction D (35 µg/mL), or saline (control) were added to human PRP, and after incubation the platelet agonist was added. These results are representative of two different experiments.
