Table 1 In vitro and in vivo assays of BjV, heated BjV and ultrafiltration fractions.

From: Jararaca GPIb-binding protein causes thrombocytopenia during Bothrops jararaca envenomation

 

Protein (mg/mL)

SVMP (%)

SVSP (%)

LAAO (%)

Platelet count (×109/L)

Fibrinogen (mg/dL)

Hemorrhage at the site of injection§

Saline

–

–

–

–

938.5 ± 21.3 (n = 14)

202.3 ± 13.7 (n = 14)

Absent (n = 14)

BjV

0.40

100.00

100.00

100.00

333.3 ± 49.8* (n = 11)

97.7 ± 8.7* (n = 11)

++++ (n = 11)

Heated BjV

1.26

0.32

64.93

0.00

1025.0 ± 25.6# (n = 5)

101.3 ± 9.2* (n = 5)

Absent (n = 5)

UF30

0.08

0.81

0.00

0.00

897.2 ± 15.5# (n = 6)

185.6 ± 21.7# (n = 6)

Absent (n = 6)

UF50

0.13

12.70

4.33

0.00

486.3 ± 49.0*,# (n = 8)

134.1 ± 20.5* (n = 8)

Absent (n = 8)

SP50

0.17

89.90

89.70

90.51

875.8 ± 46.4# (n = 5)

85.1 ± 4.7* (n = 5)

+++ (n = 5)

  1. For in vitro assays (blue columns), the protein assay and enzymatic activities (%) of SVMP, SVSP and LAAO in heated BjV and ultrafiltration fractions were comparatively shown in regard to BjV. For in vivo assays (gray columns), these fractions were injected s.c. at the dorsum of BALB/c mice (n = 5–14), and after 3 h platelet counts, plasma fibrinogen levels and hemorrhage at the site of injection were evaluated. Results were expressed as mean ± s.e.m.
  2. *p < 0.001 when compared to mice injected with saline (negative control).
  3. #p < 0.05 when compared to mice injected with BjV (1.6 mg/kg) (positive control).
  4. §Representative pictures of local hemorrhage are shown in Fig. S1.
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