Fig. 3 | Scientific Reports

Fig. 3

From: Oncolytic alphavirus-induced extracellular vesicles counteract the immunosuppressive effect of melanoma-derived extracellular vesicles

Fig. 3

The effect of rSFV-induced EVs on tumor establishment. (A) Overall scheme of the experimental steps where B16F10 cells were pre-treated with EVs from infected (SFV EVs) or non-infected cells (NT EVs) daily for 5 days and then collected for an in vivo tumor engraftment assay or an in vitro clonogenic assay. (B) Percentage of animals free from tumor and (C) related tumor growth rate upon injection with B16F10 cells pretreated with SFV EVs or NT EVs (n = 5 for each group). (D) Frequency of colonies formed from 200 cells plated after 7 days of clonogenic assay. (E) Representative images of colonies in a well formed by B16F10 cells from different conditions. In (D,E) n = 12 replicates from 4 independent experiments. One-way ANOVA followed by Bonferroni’s multiple comparisons test was performed. Data are presented as mean values ± SD.

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