Fig. 3 | Scientific Reports

Fig. 3

From: Hypoxia-induced TPC2 transcription and glycosylation aggravates pulmonary arterial hypertension by blocking autophagy flux

Fig. 3

HIF1α transcriptionally activated TPC2 expression in PAH. SD rats were divided into control and PAH (monocrotaline) groups, and PASMCs were treated with normoxia and hypoxia. (n = 5) (A–C) HIF1α abundance in PAH rats and PASMCs was detected through western blot and IHC. (D and E) ChIP-qPCR assays for determining the binding of HIF1α, Pol II and H3K27ac to the promoter of TPC2 in PAH rats and PASMCs. (F) The interaction between HIF1α and TPC2 promoter was further evaluated by EMSA assays. (G and H) qRT-PCR and western blot analysis of HIF-1α and TPC2 expression in HIF1α-overexpressing or silencing PASMCs. *P < 0.05, **P < 0.01 and ***P < 0.001. Original blots/gels are presented in Supplementary Fig. 1–3.

Back to article page