Fig. 4
The HIF1α/TPC2 axis promoted lysosomal Ca2+ release and inhibited autophagosome-lysosome fusion in hypoxic PASMCs. PASMCs were divided to control, si-NC, and si-TPC2 groups and treated with hypoxia. (n = 3) (A) TEM examination of autophagy. Scale bar, 1 µm. (B) Measurement of lysosomal Ca2+. (C) Autophagic flux was analyzed through mRFP-GFP-LC3 transfection. Red indicates mRFP, and Green indicates GFP. Scale bar, 20 µm. (D) LC3 and p62 were analyzed via western blot. (E) Cell migration was assessed by transwell assays. *P < 0.05, **P < 0.01 and ***P < 0.001. Original blots/gels are presented in Supplementary Fig. 4–8.
