Fig. 7
Inhibition of TPC2 promoted autophagy and alleviated PAH in rats. Rats were divided into PAH, PAH + Rapamycin, PAH + Chloroquine, PAH + PNGase F and PAH + Ned-19 groups. (n = 5) (A) H&E staining of pulmonary aorta and the lung. Scale bar, 100 µm. (B) IHC staining of α-SMA, TPC2 and LC3. Scale bar, 100 µm (C) TEM examination of autophagy in the lung tissue. Scale bar, 500 nm. (D) Western blot analysis of TPC2 and its glycosylation. *P < 0.05, **P < 0.01 and ***P < 0.001. Original blots/gels are presented in Supplementary Fig. 4–8.
